Pre-clinical Work

NovoSkin optimised its BTM using in vitro cell culture and numerous large and small animal studies:

  • NovoSorb TM biocompatibility and safety (rats x120)
  • BTM Mark I Vs Integra (sheep x2, 4weeks) (published in JBCR, July/August 2009)
  • BTM Mark I Vs BTM (pig x2, 4 surgical wounds 8cm x 8cm full thickness per animal, 6 months)
  • BTM immediate and delayed split skin graft (pig x6, 4 surgical wounds 8cm x 8cm full thickness per animal 4 weeks) (presented at the 43rd ABA meeting, 2011)
  • BTM with membrane (pig x2, 4 surgical wounds 8cm x 8cm full thickness per animal 4 weeks)
  • BTM Vs Integra (pig x6, 4 surgical wounds 8cm x 8cm full thickness per animal 4 weeks)

The key requirements for BTM, an implantable device, are safety and efficacy.  The following safety studies under ISO10993 have been successfully conducted:

  • Growth Inhibition (Cytotoxicity)
  • Intracutaneous reactivity
  • Sensitisation
  • Genotoxicity (mouse Lymphoma)
  • Implantation sub chronic systemic toxicity 90 days
  • Implantation sub chronic systemic toxicity 180 days
  • Subcutaneous implantation
  • Pyrogenicity